RECONSTITUTION OF TRANSPORT PROCESSES

Factors such as cytoskeletal organization, motor composition, and post-translational modifications can affect intracellular transport on the molecular level. By reconstituting different cytoskeletal networks and transport machineries and visualizing their activity via single molecule TIRF microscopy, we can elucidate the roles that these factors and others play in the transport of cargos in different organisms.

Fimbrin Fim1 (right) actively displaces tropomyosin Cdc8 (middle) during actin filament bundling. From Christensen et al. 2017.